An assay of insulin by fibril formation from small samples of pancreas
- 1 January 1958
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 68 (1), 142-145
- https://doi.org/10.1042/bj0680142
Abstract
A chemical method for the assay of insulin in 1 g samples of pancreas tissue, utilizing the phenomenon of fibril formation, is described. Recoveries are linear and near quantitative. Although some modified insulins can form fibrils, a general protein mixture from liver, as well as albumin and glucagon, does not interfere with the assay. The levels of insulin found in the pancreas of ox, pig, sheep, calf, cat and rat were comparable with those obtained by biological assay methods. No differences in insulin yield were observed when seeding fibrils from ox, pig and sheep insulins were used. An apparatus is described for the simultaneous determination of 18 samples.Keywords
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