Cytokine Expression in Normal and Inflamed Esophageal Mucosa: A Study into the Pathogenesis of Allergic Eosinophilic Esophagitis
- 1 January 2006
- journal article
- research article
- Published by Wiley in Journal of Pediatric Gastroenterology and Nutrition
- Vol. 42 (1), 22-26
- https://doi.org/10.1097/01.mpg.0000188740.38757.d2
Abstract
We studied the expression of cytokines and inflammatory cells in normal and inflamed esophageal mucosa of children with the aim of furthering our understanding of the pathophysiology of allergic eosinophilic esophagitis (AEE). Controls and AEE patients (≥15 eosinophils/high-power field on esophageal mucosal biopsies) between the ages of 1 and 18 years were recruited. Esophageal biopsies were obtained for histologic examination, immunohistochemical studies, and cytokine analysis. Eight controls (4 males; mean age 9.99 years) and 11 AEE patients (8 males; mean age 7.15 years) were studied. mRNA expression of interferon (IFN)-γ, interleukin (IL)-4, IL-5, IL-13, eotaxin-1, eotaxin-2, eotaxin-3, and RANTES was studied. IFN-γ and IL-5 expressions were significantly up-regulated in AEE patients compared with controls. Expressions of IL-4 and IL-13 were similar between AEE patients and controls. Eotaxin-1 expression was significantly up-regulated in AEE patients, whereas eotaxin-2 was up-regulated in controls. Expression of RANTES and eotaxin-3 was similar between the two groups. There was increased staining for mast cells in AEE patients compared with controls. Our data suggests that AEE is primarily an IL-5 selective TH2 response, with a possible TH1 component, and a differential role of eosinophilic chemoattractants. The role of mast cells in the pathogenesis of AEE needs additional study.Keywords
This publication has 22 references indexed in Scilit:
- Intratracheal IL-13 induces eosinophilic esophagitis by an IL-5, eotaxin-1, and STAT6-dependent mechanism1Gastroenterology, 2003
- Treatment of allergic eosinophilic esophagitis (AEE) with oral prednisone (P) and swallowed fluticasone (F): A randomized, prospective study in childrenGastroenterology, 2003
- Idiopathic eosinophilic esophagitis is associated with a TH2-type allergic inflammatory responseJournal of Allergy and Clinical Immunology, 2001
- AsthmaNew England Journal of Medicine, 2001
- An etiological role for aeroallergens and eosinophils in experimental esophagitisJCI Insight, 2001
- Allergy and the GutInternational Archives of Allergy and Immunology, 2000
- EosinophiliaNew England Journal of Medicine, 1998
- Vertical lines in distal esophageal mucosa (VLEM): a true endoscopic manifestation of esophagitis in children?Gastrointestinal Endoscopy, 1997
- Eosinophilic esophagitis attributed to gastroesophageal reflux: Improvement with an amino acid-based formulaGastroenterology, 1995