Collagen Biochemistry of the two Layers of Carious Dentin

Abstract
Carious dentin consists of a superficial first layer and a deeper second layer. Chromatographically compared with sound dentin, the second layer showed a change in the cross-linkage of collagen with cross links partly shifting to precursors. In contrast, the first layer showed virtual disappearance of cross links indicating irreversible denaturation of collagen.