Genetic analysis of human salivary ?-amylase isozymes by isoelectric focusing

Abstract
The isozymes of human salivary α-amylase have been separated by thin-layer gel isoelectric focusing in a pH 4–8 gradient followed by a starch-iodine zymogram procedure. The normal isozyme pattern (N) consisted of five major isozymes together with a number of minor ones. In addition, seven variant isozyme phenotypes were also observed, which had a combined frequency of 11.7% in a random population of 368 individuals. Analysis of familial data is indicative of the inheritance of autosomal codominant alleles.