Rapid Slide Precipitin Microreaction of Poliomyelitis Antigens and Antisera in Agar.

Abstract

A simple and rapid technique is described for demonstrating a precipitin reaction in agar between minute amounts of poliovirus antigens and antisera. The preparations can be dried and stained and preserved indefinitely. With monkey antisera the reaction was type-specific, while human sera were encountered which exhibited the precipitating antibody in the absence of readily demonstrable neutralizing antibody for the same type of poliovirus. Sera from patients with poliomyelitis and human gamma globulin regularly exhibited the precipitin reaction.