Regulated Pumilio-2 binding controls RINGO/Spy mRNA translation and CPEB activation
Open Access
- 15 January 2006
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 20 (2), 199-209
- https://doi.org/10.1101/gad.1383106
Abstract
CPEB is a sequence-specific RNA-binding protein that controls the polyadenylation-induced translation of mos and cyclin B1 mRNAs in maturing Xenopus oocytes. CPEB activity requires not only the phosphorylation of S174, but also the synthesis of a heretofore-unknown upstream effector molecule. We show that the synthesis of RINGO/Spy, an atypical activator of cyclin-dependent kinases (cdks), is necessary for CPEB-directed polyadenylation. Deletion analysis and mRNA reporter assays show that a cis element in the RINGO/Spy 3′UTR is necessary for translational repression in immature (G2-arrested) oocytes. The repression is mediated by 3′UTR Pumilio-Binding Elements (PBEs), and by its binding protein Pumilio 2 (Pum2). Pum2 also interacts with the Xenopus homolog of human Deleted for Azoospermia-like (DAZL) and the embryonic poly(A)-binding protein (ePAB). Following the induction of maturation, Pum2 dissociates not only from RINGO/Spy mRNA, but from XDAZL and ePAB as well; as a consequence, RINGO/Spy mRNA is translated. These results demonstrate that a reversible Pum2 interaction controls RINGO/Spy mRNA translation and, as a result, CPEB-mediated cytoplasmic polyadenylation.Keywords
This publication has 47 references indexed in Scilit:
- Differential Phosphorylation Controls Maskin Association with Eukaryotic Translation Initiation Factor 4E and Localization on the Mitotic ApparatusMolecular and Cellular Biology, 2005
- Symplekin and xGLD-2 Are Required for CPEB-Mediated Cytoplasmic PolyadenylationCell, 2004
- Extensive Association of Functionally and Cytotopically Related mRNAs with Puf Family RNA-Binding Proteins in YeastPLoS Biology, 2004
- Progesterone and insulin stimulation of CPEB-dependent polyadenylation is regulated by Aurora A and glycogen synthase kinase-3Genes & Development, 2004
- Spy1 Interacts with p27Kip1 to Allow G1/S ProgressionMolecular Biology of the Cell, 2003
- A Novel Mechanism for Activation of the Protein Kinase Aurora ACurrent Biology, 2003
- Dissolution of the maskin-eIF4E complex by cytoplasmic polyadenylation and poly(A)-binding protein controls cyclin B1 mRNA translation and oocyte maturationThe EMBO Journal, 2002
- RNP Localization and Transport in YeastAnnual Review of Cell and Developmental Biology, 2001
- A novel embryonic poly(A) binding protein, ePAB, regulates mRNA deadenylation in Xenopus egg extractsGenes & Development, 2001
- Speedy: a novel cell cycle regulator of the G2/M transitionThe EMBO Journal, 1999