Amplification of single-strand DNA binding protein in Escherichis coli

Abstract

An E . coli strain containing a recombinant plasmid carrying the E . coli ssbA ⁺ gene has been shown to produce 12 to 15 fold increased amounts of single-strand DNA binding-protein relative to wild-type strains. In addition, a λ transducing phage carrying the E . coli uvrA ⁺ gene has been shown to also carry the ssbA⁺ gene and to be capable of producing increased amounts of binding protein.