Association of Mouse Major Histocompatibility and Rauscher Murine Leukaemia Virus Envelope Glycoprotein Antigens on Leukaemia Cells and their Recognition by Syngeneic Virus‐Immune‐Cytotoxic T‐Lymphocytes

Abstract

Physical association was measured between gp70, the envelope glycoprotein of Rauscher murine leukemia virus (R-MLV), and serologically defined H-2 antigens on the surface of R-MLV transformed C57BL/6 (H-2DbKb) mouse leukemia cells (RBL-5A). Capping and patching with antisera against H-2Db caused specific co-capping and co-patching of R-MLV gp70 antigens as seen by fluorescence microscopy. Despite the physical proximity of R-MLV gp70 and H-2Db antigens, high titer .alpha.R-MLV gp70 sera had no effect in blocking syngeneic T [thymus derived] lymphocyte-mediated cytolysis of RBL-5A cells, whereas .alpha.H-2Db sera were effective.