Ion contents and other properties of isolated cells from dog tracheal epithelium
- 31 October 1981
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Cell Physiology
- Vol. 241 (5), C184-C192
- https://doi.org/10.1152/ajpcell.1981.241.5.c184
Abstract
We have developed a preparation of isolated cells from dog tracheal mucosa. The three major cell types of the intact epithelium (ciliated, secretory, and basal) are present in approximately the same proportions as in the intact tissue. These cells show high viability as judged by exclusion of vital dyes, high O2 consumption, and incorporation of amino acids into protein. The intracellular ion contents (in mmol/l cell H2O) are [K]i, 150; [Na]i, 20; and [Cl]i, 50. Ouabain (10(-4) M) causes a rise in [Na]i and a reciprocal loss of intracellular K.This publication has 9 references indexed in Scilit:
- Localization of Na pumps in the tracheal epithelium of the dog.The Journal of cell biology, 1979
- The Cells of the Pulmonary Airways1,2American Review of Respiratory Disease, 1977
- Basolateral plasma membrane localization of ouabain-sensitive sodium transport sites in the secretory epithelium of the avian salt glandThe Journal of cell biology, 1977
- Measures of viability in isolated cellsBiochemical Medicine, 1975
- Intracellular Cl concentrations and influxes across the brush border of rabbit ileumAmerican Journal of Physiology-Legacy Content, 1973
- Evidence for the Heterogeneous Distribution of Chloride in the Barnacle MuscleCanadian Journal of Physiology and Pharmacology, 1971
- Electron microscopic observations of the bronchial epithelium of dogs: I. Control DogsExperimental and Molecular Pathology, 1968
- Oxygen consumption and active transport in separated renal tubulesAmerican Journal of Physiology-Legacy Content, 1962
- A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acidBiochemical Journal, 1956