An automated enzymic micromethod for the measurement of fat in human milk
- 1 August 1987
- journal article
- research article
- Published by Cambridge University Press (CUP) in Journal of Dairy Research
- Vol. 54 (4), 487-492
- https://doi.org/10.1017/s0022029900025693
Abstract
Summary Most (98%) of the fat in human milk is present as triglycerides. This paper describes the use of a clarification procedure that enables the level of human milk fat to be determined by measurement of glycerol released by enzymic hydrolysis of triglycerides. The method requires only 10–50 μl milk, thus presenting a possible technique for work with small mammals, and is suitable for use with autoanalysers, permitting rapid sample throughput.This publication has 13 references indexed in Scilit:
- STATISTICAL METHODS FOR ASSESSING AGREEMENT BETWEEN TWO METHODS OF CLINICAL MEASUREMENTThe Lancet, 1986
- A procedure for the complete clarification of milk of various species and its suitability for use with colorimetric measurementsJournal of Dairy Research, 1984
- Methods of Lipid AnalysisJournal of Pediatric Gastroenterology and Nutrition, 1984
- Effect of temperature on creamatocrit method.BMJ, 1983
- Changes in the Lipids of Human Milk from 2 to 16 Weeks PostpartumJournal of Pediatric Gastroenterology and Nutrition, 1982
- Evaluation of lactational performance of Navajo womenThe American Journal of Clinical Nutrition, 1981
- Creamatocrit Procedure Versus Triglyceride Analysis: A Comparison of Methods for the Determination of Human Milk Fat in Epidemiological StudiesInternational Journal of Food Sciences and Nutrition, 1979
- Creamatocrit: simple clinical technique for estimating fat concentration and energy value of human milk.BMJ, 1978
- Structure and Synthesis of Milk Fat. X. Characterization of the Major Hydroxy Compounds in Milk LipidsJournal of Dairy Science, 1972
- Spectrophotometric Determination of Protein and Fat in Milk SimultaneouslyJournal of Dairy Science, 1970