Determination of Enantiomeric Homogeneity (Optical Purity) of Cyclophosphamide by Nuclear Magnetic Resonance Spectroscopy 2

Abstract

The enantiomeric homogeneity of resolved samples of the chiral anticancer drug cyclophosphamide was evaluated directly by ¹H and ³¹P nuclear magnetic resonance spectroscopy with the use of the optically active shift reagent tris-[3-(trifluoromethylhydroxymethylene)-d-camphorato]europium(III). These measurements, in concert with optical rotatory dispersion spectroscopy, established that, for optically pure cyclophosphamide, |α_D|=2.3±0.2°.