A Simple Two‐step Isolation Procedure for Human and Bovine Antithrombin II/III (Heparin Cofactor): a Comparison of Two Methods
- 1 October 1975
- journal article
- research article
- Published by Wiley in British Journal of Haematology
- Vol. 31 (2), 233-243
- https://doi.org/10.1111/j.1365-2141.1975.tb00853.x
Abstract
Human and bovine antithrombin II/III have been isolated by a simple procedure essentially using only affinity chromatography on heparin-agarose and polyethylene glycol precipitation. An additional ammonium sulphate step removes polyethylene glycol and trace contaminants. The final preparation is homogeneous by SDS-, disc- and agar slide electrophoresis. End group analysis of human antithrombin II/III shows histidine as the N-terminal amino acid. The pure preparation showed both progressive antithrombin activity and heparin cofactor activity.Keywords
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