A new technique for the visualization of the cytoskeleton in cultured fibroblasts with Coomassie blue R250

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Abstract
Staining of fibroblast monolayers with Coomassie brilliant blue R250 provides a relatively easy and fast method for visualization of cytoskeletal elements. Staining of detergent-extracted monolayers improves resolution and provides preparations comparable in definition to those obtained by specific immunofluorescence.