Subunit composition, crystallization and preliminary crystallographic studies of the Desulfovibrio gigas aldehyde oxidoreductase containing molybdenum and [2Fe‐2S] centers
Open Access
- 1 August 1993
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 215 (3), 729-732
- https://doi.org/10.1111/j.1432-1033.1993.tb18085.x
Abstract
The Desulfovibrio gigas aldehyde oxidoreductase contains molybdenum bound to a pterin cofactor and [2Fe‐2S] centers. The enzyme was characterized by SDS/PAGE, gel‐filtration and analytical ultracentrifugation experiments. It was crystallized at 4°C, pH 7.2, using isopropanol and MgCl2 as precipitants. The crystals diffract beyond 0.3‐nm (3.0‐Å) resolution and belong to space group P6122 or its enantiomorph, with cell dimensions a=b= 14.45 nm and c= 16.32 nm. There is one subunit/asymmetric unit which gives a packing density of 2.5 × 10−3 nm3/Da (2.5 Å3/Da), consistent with the experimental crystal density, p= 1.14 g/cm3. One dimer (approximately 2 × 100 kDa) is located on a crystallographic twofold axis.Keywords
This publication has 20 references indexed in Scilit:
- Crystallographic structure and functional implications of the nitrogenase molybdenum–iron protein from Azotobacter vinelandiiNature, 1992
- Structural Models for the Metal Centers in the Nitrogenase Molybdenum-Iron ProteinScience, 1992
- Mössbauer study of the native, reduced and substrate‐reacted Desulfovibrio gigas aldehyde oxido‐reductaseEuropean Journal of Biochemistry, 1992
- Enzymes depending on the pterin molybdenum cofactor: sequence families, spectroscopic properties of molybdenum and possible cofactor-binding domainsBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1991
- Pre‐ionization effects in the mass spectrometry of some azoxybenzenesJournal of Mass Spectrometry, 1988
- Determination of the Protein Content of Crystals Formed byMastigocladus laminosusC-Phycocyanin,Chroomonas spec.Phycocyanin-645 and Modified Human Fibrinogen Using an Improved Ficoll Density Gradient MethodBiological Chemistry Hoppe-Seyler, 1985
- Molybdenum EXAFS of the desulfovibrio gigas MO(2Fe-2S) protein—structural similarity to “desulfo” xanthine dehydrogenaseJournal of Inorganic Biochemistry, 1984
- The Reactions and the Structures of Molybdenum Centers in EnzymesPublished by Wiley ,1980
- A molybdenum-containing (2Fe, 2S) protein from desulphovibrio gigas, a sulphate reducerJournal of the Less Common Metals, 1977
- A molybdenum-containing iron-sulphur protein from desulphovibrio gigasBiochemical and Biophysical Research Communications, 1976