Human T Lymphocyte Receptors for Sheep Erythrocytes: Conditions for Binding including Inhibition by Cytochalasin B

Abstract

Requirements for binding of sheep erythrocytes (E) to human thymus-derived (T) lymphocytes were studied. The drug cytochalasin B (CB) was found to inhibit reversibly activity of the lymphocyte receptor for E. Similarly, binding was inhibited by preincubation of E with CB; the effects of CB on lymphocytes and E may be related to inhibition of microfilament function. Colchicine and vinblastine did not inhibit binding. Binding was temperature dependent but temperature dependence was reversed by Vibrio cholera neuraminidase. T lymphocyte binding of E is a physiologically active process requiring energy and intact membrane functions.