Hemolysis of Human Erythrocytes by a Sulfhydryl Inhibitor, p-Chloromercuribenzoic Acid.

Abstract

Erythrocytes incubated at 37C in p-chloromecuribenzoic acid hemolyzed. This action was prevented by prior reaction with -SH groups. Concentration of p-chloromecuribenzoic acid and temperature influenced the rate of hemolysis. Reaction once started was variously influenced by washing of cells and adding -SH groups to the suspension. Sulfhydryl groups were demonstrated in the soluble fraction of hemolyzed cell but not in the fraction containing the stroma.