Expression of Constitutively Active α-PAK Reveals Effects of the Kinase on Actin and Focal Complexes
- 1 March 1997
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 17 (3), 1129-1143
- https://doi.org/10.1128/mcb.17.3.1129
Abstract
Optimizing methods for liquid chromatography coupled to mass spectrometry (LC-MS) is a non-trivial task. Here we present rawDiag, a software tool supporting rational method optimization by providing MS operator-tailored diagnostic plots of scan level metadata. rawDiag is implemented as R package and can be executed on the command line, or through a graphical user interface (GUI) for less experienced users. The code runs platform independent and can process a hundred raw files in less than three minutes on current consumer hardware as we show by our benchmark. In order to demonstrate the functionality of our package, we included a real-world example taken from our daily core facility business.Keywords
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