Rapid Microprocedure for Extraction of Plasmid DNA from Staphylococcus aureus

Abstract

A rapid micromethod for extraction of plasmid DNA from S. aureus in quantities sufficient for restriction endonuclease analysis was developed. The method of fingerprinting is useful for identification of epidemic strains in the clinical setting and is more reliable than frequently used determinations of plasmid MW, particularly when small numbers of plasmids are involved.