Renal and hepatic excretions of phenol red and Bromsulphalein in the dog

Abstract

Effects of acetate, 2,4-dinitrophenol (DNP), Diodrast, dehydrocholic acid, and Na taurocholate on the urinary and biliary excretions of phenol red (PSP) and Bromsulphalein (BSP) were studied in 36 anesthetized dogs. Acetate increased the urinary excretion of both dyes as well as the biliary excretion of PSP whereas it had no effect on the biliary excretion of BSP. 2,4-DNP suppressed the urinary excretion of both dyes while it greatly increased the biliary excretion of PSP which was accompanied by an increase in the bile flow. However, the biliary excretion of BSP was increased only slightly by 2,4-DNP even though bile flow increased greatly. Diodrast, dehydrocholic acid, and Na taurocholate uniformly reduced both urinary and biliary excretions of PSP and BSP. Although some of these effects could be related to a corresponding change in bile flow, there was no consistent parallelism between the changes in dye excretion and in urine or bile flow. On the basis of these results, it is postulated that both the kidney and liver secrete PSP and BSP through a similar transport system. The energy dependence of this system could not be clearly ascertained from the present study.