Blood protein‐polymer adsorption: Implications for understanding complement‐mediated hemoincompatibility
- 11 February 2011
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research Part A
- Vol. 97A (1), 74-84
- https://doi.org/10.1002/jbm.a.33030
Abstract
The aim of this study was to create polymeric materials with known properties to study the preconditions for complement activation. Initially, 22 polymers were screened for complement activating capacity. Based on these results, six polymers (P1–P6) were characterized regarding physico–chemical parameters, for example, composition, surface area, pore size, and protein adsorption from human EDTA-plasma. P2, P4, and reference particles of polystyrene and polyvinyl chloride, were hydrophobic, bound low levels of protein and were poor complement activators. Their accessible surface was limited to protein adsorption in that they had pore diameters smaller than most plasma proteins. P1 and P3 were negatively charged and adsorbed IgG and C1q. A 10-fold difference in complement activation was attributed to the fact that P3 but not P1 bound high amounts of C1-inhibitor. The hydrophobic P5 and P6 were low complement activators. They selectively bound apolipoproteins AI and AIV (and vitronectin), which probably limited the binding of complement activators to the surface. We demonstrate the usefulness of the modus operandi to use a high-throughput procedure to synthesize a great number of novel substances, assay their physico–chemical properties with the aim to study the relationship between the initial protein coat on a surface and subsequent biological events. Data obtained from the six polymers characterized here, suggest that a complement-resistant surface should be hydrophobic, uncharged, and have a small available surface, accomplished by nanostructured topography. Additional attenuation of complement can be achieved by selective enrichment of inert proteins and inhibitors. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: , 2011.Keywords
This publication has 35 references indexed in Scilit:
- Synthesis and characterization of injectable composites of poly[D,L‐lactide‐co‐(ε‐caprolactone)] reinforced with β‐TCP and CaCO3 for intervertebral disk augmentationJournal of Biomedical Materials Research Part B: Applied Biomaterials, 2010
- Complement anaphylatoxin C5a contributes to hemodialysis-associated thrombosisBlood, 2010
- Recognition, Neutralization, and Clearance of Target Peptides in the Bloodstream of Living Mice by Molecularly Imprinted Polymer Nanoparticles: A Plastic AntibodyJournal of the American Chemical Society, 2010
- Can cells and biomaterials in therapeutic medicine be shielded from innate immune recognition?Trends in Immunology, 2009
- Hirudin versus heparin for use in whole blood in vitro biocompatibility modelsJournal of Biomedical Materials Research Part A, 2008
- On the mechanisms of biocompatibilityBiomaterials, 2008
- Nanoporesize affects complement activationJournal of Biomedical Materials Research Part A, 2008
- Analysis of proteins eluted from hemodialysis membranesJournal of Biomaterials Science, Polymer Edition, 1991
- Differences in the hydrophobic properties of discrete alpha-helical domains of rat and human apolipoprotein A-IVBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1987
- Vitronectin—A major cell attachment-promoting protein in fetal bovine serumExperimental Cell Research, 1985