The cDNAs coding for the α‐ and β‐subunits of Xenopus laevis casein kinase II
- 10 February 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 297 (3), 280-284
- https://doi.org/10.1016/0014-5793(92)80556-v
Abstract
Using a λgt10 cDNA library obtained from Xenopus laevis oocytes and probes derived from the known sequences of the human and Drosophila genes, a cDNA coding for the α-subunit of the X. laevis casein kinase II was isolated. The coding sequence of this clone determines a polypeptide of 350 amino acids. The X. laevis sequence is 98% identical to the human and rat proteins in the first 323 amino acids. Using the polymerase chain reaction to generate a 370-nucleotide-long probe, it was possible to clone and sequence a cDNA of 900 nucleotides that coded for the X. laevis β-subunit of casein kinase II. The derived protein sequence is 215 amino acids long and again shows an extraordinary degree of conservation with other species.Keywords
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