Influence of Zona Pellucida Thickness of Human Embryos on Clinical Pregnancy Outcome Following In Vitro Fertilization Treatment
- 1 January 2000
- journal article
- research article
- Published by Springer Nature in Journal of Assisted Reproduction and Genetics
- Vol. 17 (6), 323-328
- https://doi.org/10.1023/a:1009453011321
Abstract
Purpose: To evaluate the correlation between the degree ofzona pellucida thickness variation (ZPTV) of transferredembryos with identical morphologies and subsequent clinicalpregnancy rates during 141 intracytoplasmic sperminjection (ICSI) treatment cycles. Methods: A total of 141 women participating in the studygroup comprising mostly male factor infertilities and somerepeat in vitro fertilization (IVF) failures were transferred,2 days after ovum pickup, with two to three embryos withidentical grades created by fertilization with ICSI. Allselected embryos were subjected to zona pellucida thicknessmeasurements immediately prior to transfer using a computerizedembryo measurement program from videocinematographyrecordings. Results: A total of 326 identical-grade transferredpre-embryos resulted in 70 clinical pregnancies with live-bornimplantation rate of 27.6%. A highly significant correlationwas observed between ZPTV of transferred embryos andthe IVF outcome with 77.1% and 83.64% of the clinicalpregnancies resulting from transferred embryos with ZPTVvalues greater than 20 and 25, respectively. The mean ZPTVvalues for 70 conceptual cycles and 62 nonconceptual cycleswere 28 ± 6.43 and 17.85 ± 8.11, respectively. No significantcorrelation between ZP thickness and number of blastomeresin the transferred embryos was evident, though embryoswith better scores had significantly thinner zonae and higherZPTV values. Though average zona thickness of embryosdeclined with age, the mean ZPTV value for women lessthan 30 years old was significantly higher (25.84 ± 8.57)as compared with those from women older than 35 years(20.72 ± 8.45). Conclusions: The degree of ZPTV of the transferred embryosexhibits a strong correlation with clinical pregnancy outcome following IVF treatment. This potentially reliable indicatorof IVF success rate could be used as a criteria forembryo selection during clinical transfers.Keywords
This publication has 20 references indexed in Scilit:
- Zona pellucida thickness variation and occurrence of visible mononucleated blastomeres in preembryos are associated with a high pregnancy rate in IVF treatmentJournal of Assisted Reproduction and Genetics, 1998
- Embryo morphology or cleavage stage: how to select the best embryos for transfer after in-vitro fertilizationHuman Reproduction, 1997
- Analysis of the human zona pellucida during culture: Correlation with diagnosis and the preovulatory hormonal environmentJournal of Assisted Reproduction and Genetics, 1997
- Fertilization and early embryology: Intracytoplasmic sperm injection does not overcome an oocyte defect in previous fertilization failure with conventional in-vitro fertilization and normal spermatozoaHuman Reproduction, 1996
- Effects of the size and number of zona pellucida openings on hatching and trophoblast outgrowth in the mouse embryoMolecular Reproduction and Development, 1991
- Assisted hatching of human embryosJournal of Assisted Reproduction and Genetics, 1991
- Videocinematography of fresh and cryopreserved embryos: a retrospective analysis of embryonic morphology and implantationFertility and Sterility, 1989
- Hyperstimulation: the need for cryopreservation of embryosHuman Reproduction, 1988
- Prognostic value of morphologic characteristics of cryopreserved embryos: a study using videocinematographyFertility and Sterility, 1988
- Sperm morphologic features as a prognostic factor in in vitro fertilizationFertility and Sterility, 1986