A gene fusion that localises the penicillin‐binding domain of penicillin‐binding protein 3 of Escherichia coli
- 15 October 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 176 (1), 179-184
- https://doi.org/10.1016/0014-5793(84)80936-9
Abstract
A gene fusion that links the COOH-terminal 349 amino acids of penicillin-binding protein 3 (60 kDa) of E.coli to the NH2-terminus of β-galactosidase has been constructed. The fusion protein (38.5 kDa) retains the ability to bind benzylpenicillin with high affinity, establishing that the penicillin-binding domain (and presumably the penicillin-sensitive transpeptidase activity) of this high molecular mass penicillin-binding protein is located on a COOH-terminal functional domain.Keywords
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