Sequencing of large double-stranded DNA using the dideoxy sequencing technique
- 1 November 1982
- journal article
- research article
- Published by Portland Press Ltd. in Bioscience Reports
- Vol. 2 (11), 907-912
- https://doi.org/10.1007/bf01114897
Abstract
The dideoxy sequencing technique has been applied to the direct sequencing of large double-stranded DNA molecules with a small single-stranded primer. For instance, the method was applied to the lambda genome, which contains 48 502 base-pairs (Sanger F, Coulson AR, Hong GF, Hill D & Petersen GB, 1982, J. Mol. Biol., in press), and the coding region for gene W identified. The procedure proves useful in the sequence analysis of a large number of different mutations in a particular region and in the analysis of eukaryotic DNA cloned in plasmids, phages, and cosmids.This publication has 9 references indexed in Scilit:
- A set of synthetic oligodeoxyribonucleotide primers for DNA sequencing in the plasmid vector pBR322Gene, 1981
- Oligonucleotide directed mutagenesis of the human β-globin gene: a general method for producing specific point mutations in cloned DNANucleic Acids Research, 1981
- A method for sequencing single-stranded cloned DNA in both directionsBioscience Reports, 1981
- A system for shotgun DNA sequencingNucleic Acids Research, 1981
- Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencingJournal of Molecular Biology, 1980
- Sequence of the gene for iso-l-cytochrome c in saccharomyces cerevisiaeCell, 1979
- The relationship between function and DNA sequence in an intercistronic regulatory region in phage λNature, 1978
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- Sequence data handling by computerNucleic Acids Research, 1977