Hemosiderin. Isolation from Horse Spleen and Characterization.

Abstract

1. A method is outlined for isolating hemosiderin from horse spleen by sedimentation from salt solutions of different specific gravities. 2. The iron, nitrogen, phosphorus and ash concentration varied considerably in hemosiderin samples obtained from different spleens. Fractionation of these preparations by sedimentation in organic liquids showed that fractions could be obtained which varied in their iron concentrations from about 25 to 41% iron. 3. Hemosiderin has been found to contain hexoseamine, galactose, mannose and fucose.