Über das proteolytische System in tierischen Organen. (18. Mitteilung zur Spezifität tierischer Proteasen.)

Abstract

A proteinase of the papain type, a carboxylpolypep-tidase, an amino polypeptidase, and a dipeptidase were found in the spleen and liver as well as other animal organs. The proteolytic system of animal organs showed a closer resemblance to the enzymes of the plant cell, yeast for example, than to those of the animal digestive tract. The proteinase, kathepepsin, and car-boxylpeptidase were activated by HCN, H2S, and a natural activator, zookinase, which may be separated from the enzymes either by adsorption on kaolin, or more easily by extraction of the fresh organs with diluted alcohol or acetone. There are therefore 2 sorts of proteolytic activation in the animal organism: that of the tryptic enzyme of the gut by enterokinase, and that of the kathepeptic enzyme of the organs by zookinase. These activators are not interchangeable.