Distribution of Transmembrane Polypeptides in Freeze Fracture
- 30 March 1979
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 203 (4387), 1343-1346
- https://doi.org/10.1126/science.424755
Abstract
Human erythrocytes have been freeze-fractured, and the polypeptides associated with the separate halves of the membrane bilayer have been analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The transmembrane proteins were differentially separated by the fracture process. Although sialoglycoproteins associated with the outer half of the membrane, the anion transport protein (band 3) mainly remained with the inner half of the membrane. Well-defined fragments of the sialoglycoproteins were produced by the freeze-fracture procedure, indicating that selected covalent bonds of these transmembrane proteins were broken.This publication has 36 references indexed in Scilit:
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