Homogeneous cell populations from rabbit kidney cortex. Proximal, distal tubule, and renin-active cell isolated by free-flow electrophoresis.
Open Access
- 1 September 1977
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 74 (3), 780-788
- https://doi.org/10.1083/jcb.74.3.780
Abstract
A single-cell suspension has been prepared from rabbit kidney cortex by using a Ca-binding medium and gentle mechanical forces. The suspension was subjected to carrier-free electrophoresis, and several cell fractions were obtained. Proximal and distal tubule cell populations could be identified by their morphology. Renin-containing cells were located by means of radioimmunoassay. The morphology of the cells and their vitality (uridine incorporation) are discussed.This publication has 17 references indexed in Scilit:
- Sites of enzyme activity along the nephronKidney International, 1976
- Preparative free-flow electrophoresis for the isolation of membrane, organelle and cell fractions from rabbit kidney cortex.1976
- Isolation and subfractionation on ficoll gradients of adult rat hepatocytes. Size, morphology, and biochemical characteristics of cell fractions.The Journal of cell biology, 1975
- Subcellular localization of renin and kallikrein in rat kidney.1975
- [An enzymatic method for the isolation of tubules and cells from human kidney cortex].1975
- Criteria of Viability of Isolated Liver CellsHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1975
- [66] Isolation of renal tubulesMethods in Enzymology, 1974
- Preparation of kidney cell suspensions and respiration of kidney and liver cell suspensionsExperimental Cell Research, 1970
- Application of a Radioimmunoassay for Angiotensin I to the Physiologic Measurements of Plasma Renin Activity in Normal Human Subjects1Journal of Clinical Endocrinology & Metabolism, 1969
- A new method for the preparation of liver cell suspensionsExperimental Cell Research, 1962