COVALENT STRUCTURE OF CALF SKIN TYPE-III COLLAGEN .5. AMINO-ACID SEQUENCE OF THE CYANOGEN-BROMIDE PEPTIDE ALPHA-1(III)CB9A (POSITION 789 TO 927)

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  • 1 January 1979
    • journal article
    • research article
    • Vol. 360 (7), 851-860
Abstract
The CNBr peptide .alpha.1(III)-CB9A is 139 amino acid residues in length and occupies positions 789-927 along the .alpha.1(III) chain. Peptides necessary for the complete sequence analysis were obtained after fragmentation of .alpha.1(III)CB9B with trypsin [EC 3.4.21.1], protease from Staphylococcus aureus V8, hydroxylamine and chymotrypsin [EC 3.4.21.1]. They were separated mainly by chromatography on Sephadex G-50 and phosphocellulose and subsequently sequenced using the automated Edman degradation procedure.