Bradykinin-induced Airway Microvascular Leakage and Bronchoconstriction Are Mediated via a Bradykinin B2Receptor

Abstract

We have investigated the effects of bradykinin (Bk) antagonists on Bk-induced airway microvascular leakage and bronchoconstriction in mechanically ventilated guinea pigs by simultaneous measuring extravasation of Evans blue dye and airway opening pressure (Pao). Bradykinin (1 µg/kg intravenously) significantly increased leakage of dye in trachea, main bronchi, and intrapulmonary airways, and increased Pao, indicating airway narrowing. The selective Bk Bµ antagonist d-Arg-[Hyp³-Thi^5.8-D-Phe⁷]-Bk (NPC 349; 400 µg/kg intravenously) did not alter basal leakage, but when given 2 min before Bk, significantly Inhibited the Bk-induced plasma extravasation by 99.8% in the trachea (p < 0.05), by 75.9% in main bronchi (p < 0.05), by 83.5% in proximal intrapulmonary airways (p < 0.05), and by 91.5% in distal intrapulmonary airways (p < 0.05). NPC 349 also reduced the increase in Pao induced by Bk by 73.5% (p < 0.05) without affecting the basal Pao. However, NPC 349 had no inhibitory effect on Bk-induced leak or bronchoconstriction when given 30 min before Bk. By contrast, the B₁ antagonist des Arg⁹-Leu⁸-Bk (500 µg/kg intraveneously) had no effect on the Bk-increased plasma extravasation and Pao. We conclude that Bk increases airway plasma leakage and bronchoconstriction via activation of B₂ receptors.