Surface-Enhanced Raman Scattering from Intracellular and Extracellular Bacterial Locations
- 29 July 2008
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 80 (17), 6741-6746
- https://doi.org/10.1021/ac800838v
Abstract
While surface-enhanced Raman scattering (SERS) can increase the Raman cross-section by 4−6 orders of magnitude, for SERS to be effective it is necessary for the analyte to be either chemically bonded or within close proximity to the metal surface used. Therefore most studies investigating the biochemical constituents of microorganisms have introduced an external supply of gold or silver nanoparticles. As a consequence, the study of bacteria by SERS has to date been focused almost exclusively on the extracellular analysis of the Gram-negative outer cell membrane. Bacterial cells typically measure as little as 0.5 by 1 μm, and it is difficult to introduce a nanometer sized colloidal metal particle into this tiny environment. However, dissimilatory metal-reducing bacteria, including Shewanella and Geobacter species, can reduce a wide range of high valence metal ions, often within the cell, and for Ag(I) and Au(III) this can result in the formation of colloidal zero-valent particles. Here we report, for the first time, SERS of the bacterium Geobacter sulfurreducens facilitated by colloidal gold particles precipitated within the cell. In addition, we show SERS from the same organism following reduction of ionic silver, which results in colloidal silver depositions on the cell surface.Keywords
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