Improved medium for clonal growth of human diploid fibroblasts at low concentrations of serum protein
- 1 July 1977
- journal article
- research article
- Published by Springer Science and Business Media LLC in In Vitro Cellular & Developmental Biology - Plant
- Vol. 13 (7), 399-416
- https://doi.org/10.1007/bf02615100
Abstract
Summary A new medium (MCDB 104) has been developed which will support clonal growth of WI-38 cells at concentrations of serum protein as low as 25 μg per ml (equivalent to 0.05% serum). The principal factors responsible for reduction of the protein requirement are: (a) adjustment of all nutrient concentrations in medium F12 to experimentally determined optimum values for WI-38 cells; (b) supplementation with trace elements; (c) replacement of hypoxanthine and folic acid with adenine and folinic acid; and (d) coating of the culture surface with polylysine. Individually, many of these modifications exert only a small effect on cellular growth at reduced protein concentrations, but collectively their effect has been very substantial. Other strains of fibroblast-like human diploid cells from amniotic fluid, fetal lung and newborn foreskin also will grow at reduced concentrations of serum protein in the new medium.Keywords
This publication has 28 references indexed in Scilit:
- Effect of room fluorescent light on the deterioration of tissue culture mediumIn Vitro Cellular & Developmental Biology - Plant, 1976
- The positive control of cell proliferation by the interplay of calcium ions and cyclic nucleotides. A reviewIn Vitro Cellular & Developmental Biology - Plant, 1976
- The effect of environmental pH on the growth of normal and malignant cellsJournal of Cellular Physiology, 1973
- The requirement for biotin in mouse fibroblast L-cells cultured on serumless mediumBiochemical and Biophysical Research Communications, 1969
- Fixation spontanée de la biotine à une protéine dans le Sérum humainHelvetica Chimica Acta, 1965
- Putrescine and related amines as growth factors for a mammalian cell lineBiochemical and Biophysical Research Communications, 1963
- Biotin, B12, and other vitamin requirements of a strain of mammalian cells grown in chemically defined mediumExperimental Cell Research, 1963
- Albumin Replacement by Fatty Acids in Clonal Growth of Mammalian CellsScience, 1963
- The serial cultivation of human diploid cell strainsExperimental Cell Research, 1961
- Nutritional requirements for growth of a mouse lymphoma in cell cultureExperimental Cell Research, 1960