Characterization of the cleavage products of human serum immunoglobulin disulphide bonds. II. S-Cyano derivatives
- 1 January 1967
- journal article
- research article
- Published by CSIRO Publishing in Australian Journal of Chemistry
- Vol. 20 (6), 1265-1275
- https://doi.org/10.1071/ch9671265
Abstract
The displacement by cyanide ions of S-sulpho substituents from sulphitolysed human serum immunoglobulins yielded S-cyano derivatives, the solubility and sedimentation characteristics of which were examined with respect to pH. Exclusion chromatography gave rise to an aggregated and an unaggregated fraction in proportions similar to those found after oxidative sulphitolysis. Evidence obtained from immunoelectrophoresis, N-terminal amino acid assay, and hexose and amino acid analysis showed that both S-cyano fractions contained polypeptide chains in the same ratios as in the native protein.Keywords
This publication has 11 references indexed in Scilit:
- CHARACTERIZATION OF A HUMAN MACROGLOBULIN .I. MOLECULAR WEIGHT OF ITS SUBUNIT1965
- The Reaction of Cyanide with Bovine Serum AlbuminJournal of Biological Chemistry, 1964
- Resemblance of the Gross Arrangement of Polypeptide Chains in Reconstituted and Native γ-Globulins*Biochemistry, 1964
- Estimation of the molecular weights of proteins by Sephadex gel-filtrationBiochemical Journal, 1964
- PROPERTIES OF THE PEPTIDE CHAINS OF NORMAL AND PATHOLOGICAL HUMAN γ GLOBULINSBiochemical Journal, 1963
- THE ARRANGEMENT OF THE PEPTIDE CHAINS IN γ-GLOBULINBiochemical Journal, 1963
- STRUCTURAL UNITS OF HUMAN 7S GAMMA GLOBULIN *JCI Insight, 1960
- IMMUNOLOGICAL STUDIES OF HUMAN γ-GLOBULINThe Journal of Experimental Medicine, 1960
- The heat precipitation of Bence-Jones proteins. I. Optimum conditionsArchives of Biochemistry and Biophysics, 1959
- Thermodynamic Considerations of Protein Reactions.1,2 I. Modified Reactivity of Polar GroupsJournal of the American Chemical Society, 1954