Chromosomal mapping of the simian sarcoma virus onc gene analogue in human cells.

Abstract

The primate cell-derived transforming gene (v-sis) of simian sarcoma virus (SSV) is represented as a single copy marker within cellular DNA of mammalian species including human. The human analog of v-sis can be distinguished from its rodent counterparts by Southern blotting analysis of EcoRI-restricted DNA. By testing for the presence of the human v-sis-related fragment, c-sis (human), in somatic cell hybrids possessing varying numbers of human chromosomes and segregants of such hybrids, it was possible to assign c-sis to human chromosome 22.