INHIBITION BY 2 DIPHOSPHONATES OF BONE LYSIS IN TUMOR-CONDITIONED MEDIA

Abstract

Neonatal mouse calvaria were cultured in sterile conditions in different media. Osteolysis was estimated by the amount of 45Ca released from bone to medium, the mice being given injections of 45Ca on their day of birth. An increase bone lysis is observed when clavaria are incubated in the same medium conditioned from cultured [human] tumor fragments. This effect is significantly decreased when the mice have been treated with ethanehydroxydiphosphonate or dichloromethylenediphosphonate. These experiments represent a 1st step in estimating the potential effects of different drugs on malignant osteolysis.