Proteomic Analysis of Phagocytosis in the Enteric Protozoan Parasite Entamoeba histolytica
Open Access
- 1 April 2005
- journal article
- research article
- Published by American Society for Microbiology in Eukaryotic Cell
- Vol. 4 (4), 827-831
- https://doi.org/10.1128/ec.4.4.827-831.2005
Abstract
Proteomic analysis of phagosomes isolated from Entamoeba histolytica by liquid chromatography and mass spectrometry identified 85 proteins involved in surface recognition, actin cytoskeleton rearrangement, vesicular trafficking, and degradation. Phagosome localization of representative proteins was verified by immunofluorescence assay. This study should provide a basis for molecular identification and characterization of phagosome biogenesis.Keywords
This publication has 46 references indexed in Scilit:
- Rab5-associated Vacuoles Play a Unique Role in Phagocytosis of the Enteric Protozoan Parasite Entamoeba histolyticaJournal of Biological Chemistry, 2004
- The Intestinal Protozoan ParasiteEntamoeba histolyticaContains 20 Cysteine Protease Genes, of Which Only a Small Subset Is Expressed during In Vitro CultivationEukaryotic Cell, 2003
- Apoptotic Killing and Phagocytosis of Host Cells by the ParasiteEntamoeba histolyticaInfection and Immunity, 2003
- Endoplasmic Reticulum-Mediated Phagocytosis Is a Mechanism of Entry into MacrophagesCell, 2002
- Intermediate Subunit of the Gal/GalNAc Lectin of Entamoeba histolytica Is a Member of a Gene Family Containing Multiple CXXC Sequence MotifsInfection and Immunity, 2001
- An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein databaseJournal of the American Society for Mass Spectrometry, 1994
- Entamoeba histolytica: erythrophagocytosis, collagenolysis, and liver abscess production as virulence markersTransactions of the Royal Society of Tropical Medicine and Hygiene, 1992
- Rat and human colonic mucins bind to and inhibit adherence lectin of Entamoeba histolytica.Journal of Clinical Investigation, 1987
- Virulence of Entamoeba histolytica trophozoites. Effects of bacteria, microaerobic conditions, and metronidazole.The Journal of Experimental Medicine, 1984
- Entamoeba histolytica. Phagocytosis as a virulence factor.The Journal of Experimental Medicine, 1983