Estimation of Creatine Phosphokinase as an Additional Method for Identification of Seminal Stains

Abstract

The difficulties inherent in the identification of seminal stains in the absence of demonstrable spermatozoa are recalled. The enzyme creatine phosphokinase is present in high concentrations in seminal fluid and it is readily detected in the human ejaculate even in cases of azoospermia. The enzyme can be detected in high concentration in dried stains for at least six months. The levels of enzyme in seminal fluid are greatly in excess of those found in any other body fluid. It is suggested that estimation of the enzyme level in dried stains is a valuable addition to existing methods used when attempting to prove that a stain on clothing is seminal in origin.