A plate assay technique for inositol in yeast

Abstract

The assay of inositol in yeast was studied using a Petri plate technic and Saccharomyces carlsbergensis as the test organism. Incubation was overnight at 28[degree] to 30[degree]C. The plate method is capable of detecting as little as 20 [mu]g. of inositol per ml., but for greater precision the extracts should contain at least 60 [mu]g./ml., equivalcnt to 0.9 to 1 mg. of inositol/g. of original sample. Yeast contains about 3 mg. of inositol/g. The method is rapld and simple.