Effects of E5a and E7 genes of human papillomavirus type 11 on immortalized human epidermal keratinocytes and NIH 3T3 cells
- 1 March 1994
- journal article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 138 (1-2), 177-185
- https://doi.org/10.1007/bf01310049
Abstract
The E5a gene of HPV-11 expressed in NIH 3T3 cells led to tumorigenesis in nude mice; whereas when expressed in keratinocytes, E5a induced anchorage independent growth, but was nontumorigenic in nude mice. The E7 gene of HPV-11 expressed NIH 3T3 cells led to tumors in nude mice and morphological transformation, but not anchorage independent growth. Keratinocytes expressing the E7 gene induced colony formation in soft agarose, but not tumorigenesis in nude mice. Comparison of transforming activities of HPV-11 E5a and E7 genes of HPV-11 showed that the efficiency of cell transformation by E7 was weaker than that of E5a.Keywords
This publication has 16 references indexed in Scilit:
- E5a gene of human papillomavirus type 11 is required for initiation but not for maintenance of transformation in NIH 3T3 cellsJournal of General Virology, 1994
- Construction of site-specific mutants of E5A protein of HPV-11 using the polymerase chain reaction and a single mutant primerJournal of Virological Methods, 1993
- Comparative Study on E6 and E7 Genes of Some Cutaneous and Genital Papillomaviruses of Human Origin for Their Ability to Transform 3Y1 CellsVirology, 1993
- Co-transformation by Human Papillomavirus Types 6 and 11Journal of General Virology, 1990
- Detection by antibody probes of human papillomavirus type 6 E5 proteins in respiratory papillomataJournal of Medical Virology, 1989
- Differentiation-linked human papillomavirus types 6 and 11 transcription in genital condylomata revealed by in situ hybridization with message-specific RNA probesVirology, 1989
- The Human Papilloma Virus-16 E7 Oncoprotein Is Able to Bind to the Retinoblastoma Gene ProductScience, 1989
- Differential effects of human papillomavirus type 6, 16, and 18 DNAs on immortalization and transformation of human cervical epithelial cells.Proceedings of the National Academy of Sciences, 1989
- The nucleotide sequence and genome organization of human papilloma virus type 11Virology, 1986
- Viral etiology of juvenile- and adult-onset squamous papilloma of the larynx.Proceedings of the National Academy of Sciences, 1982