APPLICATION OF A LOCALIZED HEMOLYSIN REACTION FOR SPECIFIC DETECTION OF INDIVIDUAL ANTIBODY-FORMING CELLS
Open Access
- 1 April 1964
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 119 (4), 667-684
- https://doi.org/10.1084/jem.119.4.667
Abstract
A technique is described which makes it possible to detect individual antibody-forming cells using a localized hemolysis reaction in a thickened culture medium containing sheep erythrocytes and guinea pig complement. This technique has the advantage over single cell isolation in that it is technically feasible to survey large populations in order to detect a very small active fraction. The cells can be observed continuously during the time of antibody release, and it appears that an estimate of the relative antibody-forming activity can be made from the size of the areas of lysis. Experiments with metabolic inhibitors indicate that active synthesis is occurring rather than release of preformed antibody. Some experiments on the detection of antibody other than anti-red cell antibodies are reported.Keywords
This publication has 14 references indexed in Scilit:
- DYNAMIC ASPECTS OF FORMATION OF SERUM ANTIBODY IN RABBITS - EXPONENTIAL + ARITHMETIC PHASES IN RISE OF TITER FOLLOWING REINJECTION OF SULFANILAZO BOVINE-GAMMA-GLOBULIN1964
- Biochemistry of Cancer (Metabolic Aspects)Annual Review of Biochemistry, 1963
- Plaque Formation in Agar by Single Antibody-Producing CellsScience, 1963
- MESSENGER RNA TURNOVER AND PROTEIN SYNTHESIS IN B. SUBTILIS INHIBITED BY ACTINOMYCIN DProceedings of the National Academy of Sciences, 1962
- ANTIBODY FORMATIONThe Journal of Experimental Medicine, 1962
- The Secretion of Antibody by Isolated Lymph Node CellsJournal of Biological Chemistry, 1961
- Amino Acid Incorporation into Precipitin at Different Stages in the Secondary Response to Bovine Serum AlbuminThe Journal of Infectious Diseases, 1957
- Absence of amino acid incorporation into antibody during the induction period.Published by BMJ ,1955
- MICROMETHODS FOR THE STUDY OF PROTEINS AND ANTIBODIES .3. PROCEDURE AND APPLICATIONS OF HEMAGGLUTINATION AND HEMAGGLUTINATION-INHIBITION REACTIONS WITH BIS-DIAZOTIZED BENZIDINE AND PROTEIN-CONJUGATED RED BLOOD CELLS1955
- Specific, Complement-Dependent Hemolysis of Sheep Erythrocytes by Antiserum to Azo Hapten GroupsThe Journal of Infectious Diseases, 1952