Separation of the Complement-Fixing and Early Antigens From Epstein-Barr Soluble Antigen2

Abstract

The Epstein-Barr virus-associated complement-fixing (soluble) antigen (CFSA) and the early antigen (EA) were isolated by ion-exchange chromatography from the P3HR-1 lymphoblastoid cell line activated with 5-iodo-2′-deoxyuridine for 72 hours. As expected, these two antigens were not immunologically identical. The CFSA, but not the EA, was isolated from the RAJl lymphoblastoid cell line by the same method.