DYNAMICS OF PRODUCTION AND SENSITIVITY TO CYCLOHEXIMIDE AND ACTINOMYCIN-D OF INTERFERON-INDUCING AND INTERFERON MESSENGER-RNA

Abstract

In the process of virus[Newcastle disease virus, vesicular stomatitis virus]-induced interferon production, two kinds of RNA appear in the cells [mouse and chicken bone marrow cells and chicken embryo cells]. One of them induces production by the recipient cells of interferon with the species-specificity of the latter cells (interferon-inducing RNA), and the other is translated by the recipient cells pre-treated with actinomycin D into interferon with the species-specificity of donor cell RNA (interferon mRNA). The interferon-inducing RNA appears 20-30 min after virus induction and shows maximal activity after 1 h. Its formation is not influenced by cycloheximide or actinomycin D. This RNA is assumed to be a transcriptive intermediate form of viral RNA. Interferon mRNA appears in the cells 1 h after virus induction and shows maximal activity after 6-8 h. Its synthesis is inhibited by cycloheximide and actinomycin D.