ALPHA-METHYLDOPA AND THE ERYTHROCYTE-MEMBRANE
- 1 January 1980
- journal article
- research article
- Vol. 40 (3), 554-560
Abstract
.alpha.-Methyldopa binds to human erythrocye membrane proteins. A portion of this binding is readily dissociable in SDS [sodium dodecyl sulfate], but a significant amount is very tightly bound and does not come off even under rigorous conditions. The binding is increased under oxidizing conditions and very much inhibited in the presence of reducing agents and superoxide dismutase and catalase. Hb competes with membrane peptides for .alpha.-methyldopa binding. Hb may act as a sink for the drug in the intact cell. The 1st step in the pathogenesis of Coombs positivity and hemolytic anemia may result from an alteration of a critical membrane peptide secondary to binding of the drug during normal membrane breakdown.This publication has 16 references indexed in Scilit:
- The Rh antigen system and disaggregated human erythrocyte membranesImmunochemistry, 1977
- Reconstitution of Rh (D) Antigen Activity from Human Erythrocyte Membranes Solubilized by DeoxycholateScience, 1975
- [25] Isolation of spectrin from erythrocyte membranesMethods in Enzymology, 1974
- Electrophoretic analysis of the major polypeptides of the human erythrocyte membraneBiochemistry, 1971
- Studies on Rheomelanins: I.—The Formation of Rheomelanins in Human Blood Plasma from Catecholamines, from L-Dopa and from Some of Their DerivativesArchives Internationales de Physiologie et de Biochimie, 1970
- Annotation. AUTOANTIBODY FORMATION ASSOCIATED WITH METHYLDOPA (ALDOMET) THERAPYBritish Journal of Haematology, 1969
- The Nature of the Alpha-Methyldopa Red-Cell AntibodyNew England Journal of Medicine, 1967
- INCIDENCE OF A POSITIVE DIRECT COOMBS TEST IN PATIENTS ON α-METHYLDOPAThe Lancet, 1966
- Studies on the Metabolism and Mechanism of Action of MethyldopaCirculation, 1963
- The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytesArchives of Biochemistry and Biophysics, 1963