The Genome-Linked Protein of Picornaviruses
- 1 January 1980
- journal article
- research article
- Published by S. Karger AG in Intervirology
- Vol. 13 (3), 192-199
- https://doi.org/10.1159/000149125
Abstract
Digestion of poliovirus type 2[32P]-RNA with enzymes and analysis of the products by column chromatography and paper electrophoresis provides evidence that the RNA is covalently linked to a small, basic protein, VPg, via a 5''-terminal pU residue. Digestion of the RNA with proteinase K followed by labeling of the peptidyl-RNA with the Bolton and Hunter reagent [iodinated 3-(4-hydroxyphenyl)propionic acid N-hydroxysuccinimide ester] yields 5''-labeled material suitable for rapid sequencing. The 5''-terminal sequence of poliovirion type 2 RNA was determined to be VPg-pUUAAAACAG... which is identical to the sequence at the 5''-terminus of the poliovirus type 1 genome.This publication has 13 references indexed in Scilit:
- Sequence Studies of Poliovirus RNA. IV. Nucleotide Sequence Complexities of Poliovirus Type 1, Type 2 and Two Type 1 Defective Interfering Particles RNAs, and Fingerprint of the Poliovirus Type 3 GenomeJournal of General Virology, 1979
- O4-(5'-uridylyl)tyrosine is the bond between the genome-linked protein and the RNA of poliovirus.Proceedings of the National Academy of Sciences, 1978
- Protein is linked to the 5' end of poliovirus RNA by a phosphodiester linkage to tyrosineJournal of Biological Chemistry, 1978
- The genome-linked protein of picornaviruses IV. Difference in the VPg's of encephalomyocarditis virus and poliovirus as evidence that the genome-linked proteins are virus-codedVirology, 1978
- Encephalomyocarditis virus RNA. III. Presence of a genome-associated proteinJournal of Virology, 1978
- Identification of specific fragments containing the 5′ end of poliovirus RNA after ribonuclease III digestionNucleic Acids Research, 1978
- The 5'-terminal structures of poliovirion RNA and poliovirus mRNA differ only in the genome-linked protein VPg.Proceedings of the National Academy of Sciences, 1977
- Protein covalently linked to foot-and-mouth disease virus RNANature, 1977
- 5′-Terminal nucleotide sequences of polio virus polyribosomal RNA and virion RNA are identicalNature, 1977
- Oligonucleotide mapping of picornavirus RNAs by two-dimensional electrophoresisVirology, 1976