A Method for the Simultaneous Demonstration of Mitochondria and Their Nucleoids in the Myxomycete Physarum polycephalum by Light Microscopy
- 31 December 1976
- journal article
- research article
- Published by Japan Society for Cell Biology in Cell Structure and Function
- Vol. 2 (1), 55-58
- https://doi.org/10.1247/csf.2.55
Abstract
An HCl thionine staining technique was devised for the simultaneous demonstration of in situ mitochondria and their nucleoid in the plasmodium of P. polycephalum, and for isolated mitochondria from microplasmodia of the same organism. Light microscopy of samples stained by the method revealed that most mitochondria contained a particle-or rod-shaped nucleoid, but an appreciable number of isolated mitochondria contained V-shaped mitochondrial nucleoids. Since mitochondria containing V-shaped nucleoid were larger in size, it appears that these mitochondria may be dividing mitochondria.This publication has 2 references indexed in Scilit:
- Studies on mitochondrial structure and function in Physarum polycephalum. V. Behaviour of mitochondrial nucleoids throughout mitochondrial division cycle.The Journal of cell biology, 1977
- A method of isolation of mitochondrial nucleoid of Physarum polycephalum and evidence for the presence of a basic proteinExperimental Cell Research, 1976