Die Bestimmung der Cerebroside mit Hilfe des Pulfrich-Stufenphotometers

Abstract

A pure prep. of Cerebroside "Cerebron" and a mixture of cerebrosides obtained from human brain were used. Fifty mg. of the first, and 100 mg. of the 2d prep. were dissolved in 200 cc. 96% ethyl alc. The alcoholic soln. was evaporated to a vol. of 0.5-1.0 cc, and 3 cc. 3 N H2SO4 added, and the mixture autoclaved at 110[degree]-111[degree] for 45 min. The vol. of the soln. was restored to 3 cc, the suspended fatty acids removed with CHCl3, and 1.5. cc. of the clear soln. treated with 4 cc. conc. H2SO4 and 1 cc. orcin reagent (2 g. orcin were dissolved in 50 cc. H2O, and a cold soln. of 20 cc. conc. H2SO4 in 30 cc. H2O added). The mixture was shaken for 3 min., cooled for 6 min., and the color developed detd. in the 17 mm. cell of the Pulfrich photometer, using filters S47, S50 and S53. The amt. of galactose present was detd. from standard curves, and the amt. of cerebroside originally present calculated.