Cloning of the Bacillus subtilis spoIIA and spoVA Loci in Phage 105DI:1t

Abstract
A 6.95 kb [kilobase] HindIII-generated DNA fragment from B. subtilis 168 was inserted into the DNA of phage .vphi.105DI:1t. The recombination phage k(.vphi.105DS1) contained DNA of 33.8 kb as compared with 35.2 kb for .vphi.105DI:1t and 39.2 kb for the wild-type phage. In the presence of helper phage, .vphi.105DS1 complemented spoIIA and spoVA mutations in B. subtilis.