Restoration of membrane excitability of squid giant axons by reagents activating tyrosine-tubulin ligase
- 1 March 1979
- journal article
- research article
- Published by Springer Nature in The Journal of Membrane Biology
- Vol. 50 (1), 15-22
- https://doi.org/10.1007/bf01868785
Abstract
Using squid giant axon, an experimental survey was performed on restoration of the membrane excitability which had been partially suppressed. Among reagents examined, a combination of 400mm KF, 50 μm tyrosine, 1mm ATP, 1mm Mg ions and 5 μm cAMP was found to induce the restoration of the excitability to a large extent. Further addition of a small amount of either porcine brain microtubule proteins or the squid axoplasm was found to support complete restoration. The experiments suggest that tubulin-tyrosine ligase contained in the porcine brain microtubule protein fraction or the squid axoplasm maintains the coupling between cytoskeletal structures and the plasma membrane.This publication has 15 references indexed in Scilit:
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