Sequence and interspecies transfer of an aminoglycoside phosphotransferase gene (APH) of Bacillus circulans. Self-defence mechanism in antibiotic-producing organisms
- 15 January 1986
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 233 (2), 383-393
- https://doi.org/10.1042/bj2330383
Abstract
The APH gene of a butirosin-producing Bacillus circulans was cloned and shown to be expressed in Escherichia coli and Streptomyces lividans. The gene was sequenced and a possible developmentally regulated promoter identified. Whe the deduced protein sequence was compared with those from transposon Tn5, transposon Tn903, Streptomyces fradiae, Staphylococcus aureus and Streptococcus faecalis, significant homology was found, indicating that the genes may have a common origin.This publication has 39 references indexed in Scilit:
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