PDF file - 465K, Figure S1. Chemical structures. Figure S2. Alignment of human PARP1 (hPARP1) and chicken PARP1 (gPARP1) amino acid sequences. Figure S3. Olaparib induces PARP1- and PARP2-DNA complexes in OVCAR4 and SF295 cells. Figure S4. Differential trapping of PARP1 and PARP2 by the different PARP inhibitors in human glioblastoma SF295 cells. Figure S5. PARP1- and PARP2-poisoning by 4-amino-1,8-naphthalimide (4-AN). Figure S6. Monoubiquitination of FANCD2 in response to olaparib. Table S1. DT40 isogenic mutant cell lines used in this study. Plus, Supplementary Materials and Methods